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제목 [한국생체재료학회지] 제 23권 4호 147-159
작성자 관리자 등록일 2020-01-02
첨부파일

Background: Collagen-based scaffolds reinforced with hydroxyapatite (HA) are an attractive choice for bone tissue
engineering because their composition mimics that of bone. We previously reported the development of
compression-molded collagen-HA scaffolds that exhibited high porosity, interconnected pores, and mechanical
properties that were well-suited for surgical handling and fixation. The objective of this study was to investigate
these novel collagen-HA scaffolds in combination with human adipose-derived stem cells (hASCs) as a template for
bone formation in a subcutaneous athymic mouse model.
Methods: Collagen-HA scaffolds and collagen-only scaffolds were fabricated as previously described, and a clinically
approved bone void filler was used as a control for the material. Constructs were seeded with hASCs and were pretreated
with either control or osteogenic media. A cell-free group was also included. Scaffolds were implanted
subcutaneously in the backs of athymic nude mice for 8 weeks. Mineral deposition was quantified via microcomputed
tomography. Histological and immunofluorescence images of the explants were used to analyze their
vascular invasion, remodeling and cellularity.
Results: Cell-free collagen-HA scaffolds and those that were pre-seeded with osteogenically differentiated hASCs
supported mineral deposition and vascular invasion at comparable rates, while cell-seeded constructs treated with
the control medium showed lower mineralization after implantation. HA-reinforcement allowed collagen constructs
to maintain their shape, provided improved cell-tissue-scaffold integration, and resulted in a more organized tissue
when pre-treated in an osteogenic medium. Scaffold type and pre-treatment also determined osteoclast activity
and therefore potential remodeling of the constructs.
Conclusions: The results of this study cumulatively indicate that treatment medium and scaffold composition
direct mineralization and angiogenic tissue formation in an ectopic model. The data suggest that it may be
necessary to match the scaffold with a particular cell type and cell-specific pre-treatment to achieve optimal bone
formation.

Keywords: Bone, Osteogenesis, Angiogenesis, Adipose-derived stem cell, Collagen, Hydroxyapatite, Scaffold, Ectopic
 

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